Purpose This study aimed to identify the impact of clinical nurses’ grit and nursing work environment on job enthusiasm and provide basic data for system improvement to maintain and secure nursing personnel. Methods: Data were collected online from July to August 2022 targeting 210 clinical nurses at three general hospitals located in Jeollabuk-do. Data analysis was performed using the IBM SPSS 26.0 program. Results: Job enthusiasm exhibited statistically significant differences according to age (F=5.20, p=.006) and presence of children (F=0.95, p<.001). Furthermore, job enthusiasm showed statistically significant positive correlations with grit (r=.64, p<.001) and the nursing work environment (r=.49, p<.001). The following factors were found to affect job enthusiasm: having children (β=.26, p<.001), grit (β=.46, p<.001), nursing work environment (β=.33, p<.001), and age over 40 (β=.16, p<.001). Conclusion: Based on the results of this study, it is recommended that an internal motivation strengthening program that promotes grit should be developed and applied to increase work enthusiasm among clinical nurses. Additionally, hospital organizations should prepare various policies to improve the nursing work environment.