Purpose This study’s purpose was to identify the influence of retirement expectations and readiness on retirement anxiety among middle-aged nurses. Methods The participants were 216 middle-aged nurses working at nine locations including hospitals, long-term care hospitals, general hospitals, and tertiary hospitals in B city. Data were collected from April 25 to May 13, 2022 using self-report questionnaires and analyzed using t-tests, one-way ANOVAs, Scheffé́ tests, Pearson’s correlation coefficients, and multiple regression with SPSS/WIN 28.0. Results The mean scores for retirement expectations, retirement readiness, and retirement anxiety were 3.49±0.38, 3.21±0.59, and 2.48±0.51, respectively. Retirement anxiety was found to be significantly affected by new beginnings (β=-.31, p<.001) for retirement expectations and life design readiness (β=-.27, p=.002) and, leisure readiness (β=-.27, p<.001) for retirement readiness. These variables accounted for 33.0% the retirement anxiety among middle-aged nurses. Conclusion To reduce retirement anxiety among middle-aged nurses, it is necessary to adopt an organizational approach enhancing their perceptions and using a policy alternative to make use of older nurses' extensive knowledge and experience.