Purpose Nursing care activities for patient safety are important to prevent accidents. This study aimed to examine the association between nurses’ perception of patient safety culture, willingness to report near misses, critical thinking disposition, and nursing care activities for patient safety. Methods In this cross-sectional study, data were obtained from online surveys conducted with 201 ward nurses. An independent t-test, one-way ANOVA, Pearson’s correlation coefficient test, and multiple linear regression analysis were performed using IBM SPSS/WIN 21.0. Results Perceptions of patient safety culture, willingness to report near misses, critical thinking disposition, and nursing activities for patient safety were all correlated with each other. According to the regression model, significant factors influencing nursing care activities for patient safety were medical ward (β=-.28, p=.010), critical thinking disposition (β=.27, p<.001), willingness to report near misses (β=.19, p=.004), perceptions of patient safety culture (β=.19, p=.007), and work experience (β=-.18, p=.016). Conclusion Improving nurses’ perceptions of patient safety culture, promoting the reporting of near misses, and strengthening nurses' critical thinking dispositions can foster patient-safety nursing care activities. Additionally, a tailored patient safety education program that considers nursing work experience may be appropriate for patient-safety nursing care activities.